Tmem192-ha
WebDisclosed herein are methods and compositions for modulating MFSD12 expression and activity to treat diseases such as lysosomal storage diseases, including cystinosis. Also disclosed are methods of altering skin pigmentation and methods of screening for MFSD12 modulation agents. WebTransmembrane protein 192 (TMEM192) has been previously identified in proteomic analyses of lysosomal membranes. TMEM192 does not exhibit any significant homology …
Tmem192-ha
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WebSep 29, 2024 · (A) Scheme depicting the targeting strategy for insertion of a 3 x-HA epitope at the C-terminus of the TMEM192 gene in HeLa cells. (B) Whole cell extracts from the indicated HeLa cells (CONTROL or TMEM192 HA) were immunoblotted using the indicated antibodies.(C) Immunostaining of HeLa TMEM192 HA cells with α-TMEM192, α-NPC1 as … WebYour search returned 97 TMEM192 Western Blot Antibodies across 21 suppliers. Showing 21 of 21 suppliers (97 products total) > >> Select All. Select up to 5 products from below to compare or request more information. Request Info for all products Compare. Sponsored Products MyBioSource.com TMEM192 Antibody ...
WebSep 21, 2024 · TMEM192–3×HA marks lysosomes with a triple HA epitope, enabling their immunoisolation with anti-HA antibodies. Immunofluorescence-based analyses of liver and brain sections, two tissues... WebTmem192 Constitutive Knockout Inquire CALL: 888.822.6642 + 45 70 23 04 05 (EUROPE and DENMARK) +49 214 50 68 023 (GERMANY) Design Your Own Genetically Engineered Animal Model Learn More This line is cryopreserved and requires cryorecovery. Model # Genotype Nomenclature TF4095 Inquire MGI:1920317 Model Description Mutation Image …
WebTmem192 Constitutive Knockout. start an order print this page share this page. Inquire. CALL: 888.822.6642 + 45 70 23 04 05 (EUROPE and DENMARK) +49 214 50 68 023 … WebApr 12, 2024 · (J–L) (J) Schematic description, (K) immunoblot, and (L) quantification of lysosome ESCRT components by anti-HA immunoprecipitation (LysoIP; TMEM192-3xHA) from HeLa ATG5-WT and HeLa ATG5-KO cells treated as in (F). Long exp (long exposure) and Short exp (short exposure) correspond to the same blot. Data, mean ± SE (n = 3); two …
WebApr 10, 2024 · Cells stably expressing TMEM192-3×HA were treated, rinsed once with pre-chilled PBS, and scraped off in PBS. After centrifugation, cell pellets were resuspended in 1 ml PBS supplemented with an ...
Webtagged TMEM192 (Control-Lyso cells) or 3xHA-tagged Tmem192 (HA-Lyso cells). ER, endoplasmic reticulum. ( Dto F) Purified lysosomes are intact and retain their contents. (D) Cathepsin D activity was measured in whole-cell lysates and lysosomes, and immunoprecipitates from Control-Lyso cells served as a negative control (Control IP) … concept of current in hindiWebMar 21, 2024 · GeneCards Summary for TMEM219 Gene. TMEM219 (Transmembrane Protein 219) is a Protein Coding gene. Diseases associated with TMEM219 include … eco school 7 stepsWebAug 13, 2024 · HA-TMEM192-isolated lysosomes represented a highly pure fraction as determined by the absence of markers for other cellular compartments such as … eco school action plan templateWebImage 1 of 6. ( Enlarge) Immunocytochemistry/ Immunofluorescence: TMEM192 Antibody [NBP1-93798] - Staining of human cell line U-2 OS shows localization to endosomes & lysosomes. Antibody staining is shown in green. Immunohistochemistry-Paraffin: TMEM192 Antibody [NBP1-93798] - Staining of human cerebral cortex shows strong granular … eco school auditWebJul 27, 2024 · J. Representative immunofluorescence staining of ER (CLIMP63, green) and lysosomes (TMEM192-HA, red) in FGFR3;4KO chondrocytes treated with vehicle (5% ABS) and FGF18 ( 50 ng/ml for 16 h). BafA1 was used at 100 nM for 4 h to inhibit lysosomal degradation. Quantification of relative CLIMP63 fluorescence into TMEM192-HA-positive … concept of database securityWebOct 13, 2024 · Gene expression analysis, associated with functional characterization examining neurotransmitter release and electrical recordings, support the functional identity of the iDA neurons to A9 midbrain... eco school action plan examplesWebApr 6, 2024 · This initial TFEB-independent recovery of hydrolase activity may be explained by previous reports that demonstrated that 1 mM LLOMe treatment for 2 h causes … eco school assembly