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Biotin ctp

WebDec 16, 2024 · In our design, the biotin CTP could be changed to any other biotin nucleobase depending on which nucleobase presents the most in the probe sequence to achieve optimal labeling efficiency; iii) the ratio between the forward primer and the reverse primer in the biotin-probe synthesis process was adjusted to 1:4 to allow the formation … WebBiotin-16-dCTP is a modified deoxynucleoside triphosphate (dNTP), serving as a convenient tool for the enzymatic introduction of the biotin moiety into a nucleic acid target of interest. The biotin moiety exhibits a very strong affinity for streptavidin. The strength and specificity of this interaction has been exploited in a variety of ...

CAS：17796-82-6 N-环己基硫代酞酰亚胺-德尔塔(Delta)生物试剂

WebDynabeads Streptavidin for Target Enrichment provides the following advantages: High purity —helps minimize non-specific binding. High reproducibility —achieve consistent results and can help reduce the risk of failure. High sensitivity —secure successful NGS workflow. Automation – enables high-throughput enrichment. WebBiotin-11-CTP, >95%. Life Sciences & Diagnostics A Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance … eyebrow waxing greece ny https://myorganicopia.com

Biotin-11-CTP solution, pkg of 25 μL (250 nmol; 10 mM) - Sigma-Aldrich

WebBiotin-14-CTP can be efficiently incorporated into RNA transcripts using T7, SP6, or T3 RNA polymerase and the appropriate template in the presence of ATP, GTP, UTP, and Biotin-14-CTP. The biotinylated RNA can be used as a probe in membrane-based or in situhybridizations. The amount of material provided is sufficient for up to 20 transcription ... WebApr 14, 2024 · 英文名：Biotin-PEG-DSPE DSPE-PEG-Biotin DSPE-PEG-BIO 中文名：二硬脂酰磷脂酰乙醇胺-聚乙二醇-生物素 生物素聚乙二醇磷脂;磷脂聚乙二醇生物素 磷脂PEG生物素 荧光素聚乙二醇磷脂 英文化学名：1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotin(polyethylene glycol)] CAS：385437-57-0 分子式：C142H280N5O56PS 分子 … WebApplication. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA Polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template … eyebrow waxing gone wrong

MessageAmp™ II-Biotin Enhanced Kit - Thermo Fisher Scientific

Category:Design and use of a back splicing junction probe for pulldown of ...

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Biotin ctp

Comprehensive profiling of CTP-binding proteins using a …

WebJul 4, 2024 · National Center for Biotechnology Information WebThe unique biotinylated cytidine (bis)phosphate used in this kit enables T4 RNA ligase to attach a single biotin tag on RNA probes of diverse secondary structure and lengths with > 70% efficiency. Labeling …

Biotin ctp

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WebBiotin-14-CTP is a CTP analog with biotin attached at the N 4 -position of the pyrimidine base by a 14-atom linker. Biotin-14-CTP can be efficiently incorporated into RNA … WebBiotin-14-CTP is a CTP analog with biotin attached at the N 4 -position of the pyrimidine base by a 14-atom linker. Biotin-14-CTP can be efficiently incorporated into RNA …

WebBiotin-11-CTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace CTP in the in vitro transcription reaction for RNA labeling. Double-stranded DNA ( i.e., linearized plasmid or cDNA) containing a T7, SP6, or T3 promoter serves as a template for in vitro transcription with the corresponding RNA polymerase, using ATP, GTP, CTP, UTP ... WebNov 1, 2024 · In this study, we designed a biotinylated CTP affinity probe which harbors three components: CTP, a binding moiety, which specifically recognizes the active sites of CTP-binding proteins; biotin, an enrichment moiety, which facilitates the enrichment of target proteins; γ-aminobutyric acid, a linker, which connects the enrichment moiety to …

WebA fairly wide range of concentrations of Biotin-UTP (25–60%) can be used for acceptable detection, whereas at lower concentrations the signal drops dramatically, and at higher … WebBiotin-11-CTP (Biotin-11-cytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into RNA 10 mM solution in pH 7.5 Tris-HCl buffer Store at -20°C

Web40035. Price. 553.79 USD. Size. 25 µL. Contact us. Ask 11-CTP 10 mM in pH7 5 TE 11-cytidine-5' triphosphate tetralithium salt selection audit. + Add to compare.

WebNov 1, 2024 · We successfully synthesized the biotin-tagged CTP affinity probe and used it to enrich CTP-binding proteins by two sets of competitive SILAC experiments. We … eyebrow waxing greenhillsWebOptimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine. Recommended Biotin-11 … dodge ram 1500 towingWebBiotin-11-dCTP is a deoxycytidine triphosphate (dCTP) linked to a biotine molecule via an 11-atom linker, which allows efficient interaction with avidin and streptavidin; … eyebrow waxing grand rapids miWebShelf Life: 12 months after date of delivery. Molecular Formula: C 28 H 44 N 7 O 17 P 3 S (free acid) Molecular Weight: 875.67 g/mol (free acid) Exact Mass: 875.17 g/mol (free … dodge ram 1500 towing weightWebBio-11-CTP (Biotin-11-cytidine-5’-triphosphate) can replace CTP for in vitro transcription reaction catalyzed by T3, T7 or SP6 RNA polymerases. The biotin-labeled RNA … eyebrow waxing huntersville ncWebLinearized template DNA with T7, SP6 or T3 promoter is in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP and Biotin-16-UTP, respectively. eyebrow waxing greenville ncWebFeb 23, 2004 · Biotin pull-down assays were carried out by incubating 40 μg of cytoplasmic fractions with 1 μg of biotinylated transcripts for 1 h at room temperature. Complexes were isolated with paramagnetic streptavidin-conjugated Dynabeads (Dynal, Oslo, Norway), and bound proteins in the pull-down material were analyzed by Western blotting by using ... dodge ram 1500 trailer hitch receiver